Dr. Bhawna Gupta
Assistant Professor
Ph.D, Institute of Genomics and integrative Biology, University of Delhi, India
M. Sc. Department of Zoology, University of Delhi


Email:
[email protected]

Post-Doctoral Experience

  • University of Lausanne, Switzerland
  • Ludwig Centre for Cancer Research and Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland
  • Centre for Biotechnology, Finland

Research Interest

Autoimmunity encompasses immune dysfunction that counters self, results in target tissue destruction and involves multi-organ ramification. More than seventy autoimmune disorders have been registered so far. Despite their heterogeneity, autoimmune diseases share epidemiological, etiopathogenic, and clinical features. Our understanding of the mechanisms that lead to autoimmunity is evolving and the past two decades of research has yielded rich insights into the pathogenesis of these diseases. Interplay of genetic, immunological and environmental factors are driving forces towards pathogenesis of autoimmune diseases.

It is essential to understand the biology of autoimmune disorders in a spatio-temporal manner following immune responses of different players of immune system. The current paradigms of innate and adaptive immunity highlight the role of dendritic cells, macrophages, CD8+ T cells and CD4+ T cells as major effector cells in the progression of autoimmune disorders. The phenotypic and functional differences in these cells mount an effective auto-immune response. The current interest of our lab is to identify the molecular mechanisms leading to Rheumatoid Arthritis (RA), a disabling autoimmune disease affecting more than 1% of Indian population.

Early diagnosis and treatment with disease-modifying anti-rheumatic drugs is important to control rheumatoid arthritis. However, in patients with early disease, the joint manifestations are often difficult to distinguish from other forms of inflammatory polyarthritis as distinctive signs of RA are seen primarily in patients with longstanding, poorly controlled disease. Disease remission has become the ultimate target in RA by improved treatment options. However, remission is only achieved in a minority of RA patients and sustained remission is even more difficult to reach. Therefore, following a comprehensive understanding of the disease mechanism we would like to identify better diagnostic markers and druggable targets to improve the treatment strategies.

Further, since averaging over population only masks important information, we are interested to identify biomarkers, druggable targets and rare cells at the Single Cell resolution.

Awards/Honors

  • CSIR-UGC Senior Research Scholarship, 2005.
  • CSIR-UGC Junior Research Scholarship, 2003.
  • Jawahar Lal Nehru merit cum means scholarship, University of Delhi, 2001
  • Miranda House Scholarship, University of Delhi, 2000.
  • President, Zoological society, Miranda House, University of Delhi, 2000.
  • Secretary, Zoological society, Miranda House, University of Delhi, 1999.

Selected Publications

  1. Epigenomics of autoimmune diseases. Immunology and Cell Biology Special Feature "Epigenetics and Disease" 2015.
  2. Proviral integration site for Moloney murine leukemia virus (PIM) kinases promote human T helper 1 cell differentiation. J Biol Chem. 2013 Feb 1; 288 (5): 3048-58.
  3. Identification of early gene expression changes during human Th17 cell differentiation. Blood. 2012 Jun 7; 119 (23): e151-60.
  4. Single cell analysis reveals similar functional competence of dominant and nondominant CD8 T-cell clonotypes. Proc Natl Acad Sci U S A. 2011 Sep 13; 108 (37): 15318-23.
  5. Genome-wide profiling of interleukin-4 and STAT6 transcription factor regulation of human Th2 cell programming. Immunity. 2010 Jun 25; 32 (6): 852-62.

We are interested to understand the molecular mechanisms of autoimmune disorders, especially rheumatoid arthritis, by a Systems Biology approach at the Single Cell resolution.

  1. Single cell analysis of dendritic cells in progression of rheumatoid arthritis.
  2. Role of CD8+ T cells in pathogenesis and progression of rheumatoid arthritis.
  3. Understanding the mechanisms of inflammasome mediated disease activity in rheumatoid arthritis.
  1. Dr. Kanury V S Rao, Head, DDRC, THSTI, Delhi and Group Leader, ICGEB, Delhi.
  2. Prof. Bart Deplancke, EPFL, Switzerland.
  3. Prof. Hans Acha-Orbea, UNIL, Switzerland.
  4. Dr. R David Hawkins, University of Washington, USA.
  5. Maj. Gen. (Dr.) Ved P Chaturvedi, Army Hospital, Delhi.
  6. Dr. Prasanta Padhan, KIMS, Bhubaneswar.
  7. Dr. Sunil Kumar Raghav, ILS, Bhubaneswar.
  1. Shweta Khanna
    PhD Student
    Email: [email protected]
    Project: To identify the role of Dendritic Cells in rheumatoid arthritis
    Publications: Adlakha, Y. K., Khanna, S., Singh, R., Singh, V. P., Agrawal, A., & Saini, N. (2013). Pro-apoptotic miRNA-128-2 modulates ABCA1, ABCG1 and RXRα expression and cholesterol homeostasis. Cell death & disease4(8), e780.
  2. Archana Tripathy
    PhD Student
    Email: [email protected]
    Project: To identify the role of CD8+T cells in rheumatoid arthritis
    Award: Chancellor’s Gold medal Award (2014)
  1. Single Cell Transcriptomics for Autoimmune Disorders. J Mol Gen Med; 2015, 9:4
  2. Epigenomics of autoimmune diseases. Bhawna Gupta* and R. David Hawkins. (*corresponding author). Immunology and Cell Biology Special Feature "Epigenetics and Disease" 2015.
  3. Persistence of EBV antigen-specific CD8 T cell clonotypes during homeostatic immune reconstitution in cancer patients. Iancu EM, Gannon PO, Laurent J, Gupta B, Romero P, Michielin O, Romano E, Speiser DE and Rufer N. PLoS One. 2013 Oct 25;8(10):e78686.
  4. Proviral integration site for Moloney murine leukemia virus (PIM) kinases promote human T helper 1 cell differentiation. Tahvanainen J*, Kylaniemi MK*, Gupta B, Lähdesmäki H, Kallonen T, Rajavuori A, Rasool O, Lahesmaa R. J Biol Chem. 2013 Feb 1; 288 (5): 3048-58. *equal contribution.
  5. Simultaneous coexpression of memory-related and effector-related genes by individual human CD8 T cells depends on antigen specificity and differentiation. Gupta B, Iancu EM, Gannon PO, Wieckowski S, Baitsch L, Speiser DE, Rufer N. J Immunother. 2012 Jul; 35 (6): 488-501.
  6. Identification of early gene expression changes during human Th17 cell differentiation. Tuomela S, Salo V, Tripathi SK, Chen Z, Laurila K, Gupta B, Äijö T, Oikari L, Stockinger B, Lähdesmäki H, Lahesmaa R. Blood. 2012 Jun 7; 119 (23): e151-60.
  7. Single cell analysis reveals similar functional competence of dominant and nondominant CD8 T-cell clonotypes. Speiser DE*, Wieckowski S*, Gupta B, Iancu EM, Baumgaertner P, Michielin O, Romero P, and Rufer N. *equal contribution. Proc Natl Acad Sci U S A. 2011 Sep 13; 108 (37): 15318-23.
  8. Genome-wide profiling of interleukin-4 and STAT6 transcription factor regulation of human Th2 cell programming. Elo LL*, Järvenpää H*, Tuomela S*, Raghav S*, Ahlfors H, Laurila K, Gupta B, Lund RJ, Tahvanainen J, Hawkins RD, Oresic M, Lähdesmäki H, Rasool O, Rao KV, Aittokallio T, Lahesmaa R. Immunity. 2010 Jun 25; 32 (6): 852-62. *equal contribution.
  9. S-nitrosylation of mannose binding lectin regulates its functional activities and the formation of autoantibody in rheumatoid arthritis. B Gupta, SK Raghav, HR Das. Nitric Oxide, 2008 Jun; 18(4): 266-73.
  10. Rapid and sensitive detection of autoantibody in Rheumatoid Arthritis patients by heat-mediated ELISA. P Sharma, B Gupta, SF Basir, HR Das, P Nahar. Clin Biochem, 2008; 41(1-2):97-102.
  11. Inhibition of lipopolysaccharide inducible iNOS and IL-1b expression by active compounds isolated from Ruta graveolens L. through suppression of NF-kB activation in murine macrophages. Raghav SK, Gupta B, Shrivastava A, Das HR. Euro J Pharmacol, 2007, 29; 560.
  12. Anti-MBL autoantibodies in patients with rheumatoid arthritis: prevalence and clinical significance. Bhawna Gupta, Sunil Kumar Raghav, Charu Agrawal, Rakha Hari Das, Ved Prakash Chaturvedi and Hasi Rani Das. J. Autoimmunity, 2006, Sep;27(2):125-33.
  13. Expression of TNF-a and related signaling molecules in the peripheral blood mononuclear cells of rheumatoid arthritis patients. Raghav SK, Gupta B, Agrawal C, Chaturvedi VP and Das HR. Mediat. Inflamma. 2006, Article ID 12682, pages 1-6.
  14. Altered expression and glycosylation of plasma proteins in rheumatoid arthritis. Sunil K. Raghav, Bhawna Gupta, Charu Agrawal, Ashish Saroha, Rakha H. Das, Ved P. Chaturvedi and Hasi R. Das. Glycocon. J. (2006) 23: 169-175.
  15. Anti-inflammatory effect of Ruta graveolens L. in murine macrophage cells. Raghav SK, Gupta B, Agrawal C, Goswami K, Das HR. J Ethnopharmacol. 2006 Mar 8; 104 (1-2):234-239.
  16. Association of mannose-binding lectin gene (MBL2) polymorphisms with rheumatoid arthritis in an Indian cohort of case-control samples. Gupta B, Agrawal C, Raghav SK, Das SK, Das RH, Chaturvedi VP, Das HR. J. Hum. Genet. (2005) 50: 583–591.
  17. Tumor necrosis factor-alpha microsatellite polymorphism association with rheumatoid arthritis in Indian patients. Agrawal C, Raghav SK, Gupta B, Das RH, Chaturvedi VP, Goswami K, Das HR. Arch. Med. Res. 2005; 36 (5): 555-9.

Patents applied for/accepted

  1. Method for the diagnosis of rheumatoid arthritis. Hasi Das, Bhawna Gupta, Sunil Kumar Raghav, Kalyan Goswami, Charu Agrawal and Rakha Hari Das. IPC8 Class: AG01N3353FI USPC Class: 435 71
  2. Novel anti-inflammatory compound 3-(1, 1-dimethyl-allyl)-6-hydroxy-chromen-2-one isolated from Ruta graveolens L. Hasi Das, Sunil Kumar Raghav, Bhawna Gupta. IPC8 Class: AA61K31352FI, USPC Class: 514456
  3. Novel diagnostic marker, a diagnostic kit and a method for diagnosis of rheumatoid arthritis. Hasi Das, Bhawna Gupta, Sunil Kumar Raghav, Kalyan Goswami, Charu Agrawal and Rakha Hari Das. USPC Class: 435792

SNPs  submitted

  • SNPs in the MBL gene submitted to NCBI dbSNP:  A/G  (rs12720433), C /T (rs17334270), A /G /T (rs17287498), C /T (rs1982266), C /G (rs930508), C /G (rs930507), A /G (rs13312736).

Book Chapters

  • Anti-inflammatory compounds from medicinal plant Ruta graveolens. Das HR, Raghav S, Gupta B and Das RH. Acta Hort. 2007 756, 389- 398 (2007)