Dr. Gopal Chowdhary
Assistant Professor
Ph.D. KIIT and University of Stavanger, Norway
M.Sc. Department of Botany, University of Delhi


Email : [email protected]

Postdoctoral Experience

  • Feb 2013 - Feb 2014: Center for Organelel Research (CORE), University of Stavanger,  Stavanger, Norway.
  • Master of Philosophy (M. Phil. Plant Molecular Biology & Biochemistry): University of Delhi, India

Research Interest

  1. Abiotic stress signal transduction:
    Due to immobile in nature, plants cannot escape from the environmental adversaries. To withstand them, plants have developed sophisticated tolerance mechanisms. The damage to world agriculture caused by abiotic stresses amounts to billions of dollars. According to an estimate of the International Panel on Climate Change (IPCC), the global temperature will rise up to 30C with the advent of climate change, thereby increasing the frequency and intensity of abiotic stress conditions (IPCC, 2007). The global population is expected to reach 9 billion by 2050, 60-70% of which be living in developing and underdeveloped nations. With the current trend of loss of standing crop and ever increasing population it will be very difficult to keep pace with the demand and supply of major food grains. Due to scarcity of land it is also not possible to bring more areas under cultivation. The immediate solution to this problem lies in developing stress resistant plant varieties. Here, at our laboratory we intend to study the stress tolerance mechanism primairly, mediated by peroxisomal proteins. We employ a combination of bioinformatics, genomics and proteomics approach to achieve our goal.
  2. Role of plant growth promoting rhizobacteria in abiotic stress adaptation: We are trying to isolate and characterize novel strains of PGPR from stress affected soil. Further, these isolates are being studied for their efficacy in stress adaptation of crop plants.

Awards/Honours

  1. YGGDRASIL (IS-MOBIL) Fellowship: Sept 2010-May 2011, Awarded by Research Council of Norway, Govt. of Norway, to work at Center for Organelle Research, University of Stavanger, Norway.
  2. National Eligibility Test (NET) December 2002: Awarded jointly by University Grants Commission (UGC), Government of India and Council of Scientific and Industrial Research (CSIR), Govt. of India.
  3. Graduate Aptitude Test in Engineering (GATE): Qualified, GATE 2002, Percentile score 96.42, All India Rank: 90; awarded by Indian Institute of Technology, Delhi, Govt. of India.
  4. National Scholarship under Center of Advance Study in Botany for the academic year 2001-2002 and 2002-2003, awarded by University of Delhi, Delhi, India.

Selected Publications

Full List
  1. S. Reumann, G. Chowdhary and   T. Lingner (2016).Characterization, prediction and evolution of plant peroxisomal targeting signals type 1 (PTS1s). Biochemica et Biophysica Acta (BBA) - Molecular Cell Research, 1863, (5), 790–803.
  2. Nicola S. Skoulding, Gopal Chowdhary, Mara J. Deus, Alison Baker, Sigrun Reumann and Stuart L. Warriner (2014) Experimental Validation of Plant Peroxisomal Targeting Prediction Algorithms by Systematic Comparison of In Vivo Import Efficiency and In Vitro PTS1 Binding Affinity; J Mol Biol. 2014 Dec 10. - (14) 00619-6. [Epub ahead of print].
  3. Chowdhary G., Kataya, A.R.A., Lingner, T., and Reumann, S. (2012) Non-Canonical Peroxisome Targeting Signals: Identification of Novel PTS1 Tripeptides and Characterization of Enhancer Elements by Computational Permutation Analysis. BMC Plant Biology 12, 142
  4. Chowdhary G. and Kundu C. N. (2013) Induction of a C-Repeat Binding Factor Gene by Calcium to resist Low Temperature stress in rice. Int. J. Plant Physiol. Biochem. 6 142-145.
  5. Chowdhary G. K., Deswal R., and Sopory S. K. (2004) Purification and characterization of a 3. PMA stimulated kinase and identification of PMA induced phosphorylation of a polypeptide that is dephosphorylated by low temperature in Brassica juncea. Biochem. Biophys. Res. Commun. 322: 420-427

Lab

Our lab primarily focuses on research related to plant molecular biology and plant proteomics. We work on three different plant species: model plant Arabidopsis thaliana, Oryza sativa (rice) and Lyscopersicon solanum (tomato). Our priority area is abiotic stress signal transduction with special reference to peroxisome. Peroxisomes are single membrane bound organelle, conventionally considered responsible for beta oxidation. However, with the advent of recent bioinformatics, molecular and biochemical tools newer functions have been attributed to it. With better subcellular prediction algorithms followed by fluorescent fusion protein construct analysis the peroxisomal proteome pool is increasing. Being one of the nodal centre of reactive oxygen species (ROS) metabolism in plant, it is supposed to play important role in stress tolerance as well. In our lab we use a combination of bioinformatics, molecular, proteomics and metabolomics tools to better understand the plant peroxisome biology.

Our lab is also involved in understanding the role of plant growth promoting rhizobacteria (PGPR) in abiotic stress tolerance. Here, we are using rice as a model to study the PGPR-plant interaction. Currently, we are investigating the underlying signal transduction mechanism.

Our lab is primarily funded by SERB-DST, BIRAC-DBT, Govt. of India, Govt of Ethiopia (Indo-Ethiopian Collaboration) and KIIT Deemed to be University.

Projects

  • Phytostabilization of mine tailings in the Sukinda chromite mining area: development and optimization of a PGPR (plant growth-promoting rhizobacteria) assisted technology, funded by Department of Biotechnology, Govt. of India.
  • Functional Characterization of peroxisomal polyamine oxidase mediated abiotic stress tolerance in rice, Funded by Scientific and Engineering research council (SERB), Govt. of India. Polyamines (PAs) are organic compounds having two or more amino groups. In plants they are suggested to play important role in embryogenesis, cell division, organogenesis, flowering, senescence, and also in responses to environmental stresses. Polyamine oxidases (PAO) are the class of enzymes having a crucial role in PA homeostasis in cell. PA metabolism in plant leads to production of hydrogen peroxide, which in turn produces reactive oxygen species (ROS). Peroxisomes are known to be one of the nodal centres (apart from mitochondria and chloroplast) for ROS homeostasis in cell, hence in this project we intend to study the role of peroxisomal PAO is abiotic stress acclimation.
  • Development of Novel Electromagnetic Wave Based Rapid Tissue Fixation and Processing Device: A biotechnology Ignition Grant (BIG) Scheme, funded by Biotechnology Industry Research Assistance Council (BIRAC), DBT, Govt. of India.
  • Development of TiO2 Nano-particles Impregnated Surgical Silk Suture - A Novel Approach: A biotechnology Ignition Grant (BIG) Scheme, funded by Biotechnology Industry Research Assistance Council (BIRAC), DBT, Govt. of India.

Collaborators

  • Dr. Ram Singh Purty, University School of Biotechnology, Guru Gobind Singh Indraprastha University, Sec -16C, Dwaraka, New Delhi - 110078, India
  • Dr. Abikshyeet Panda, Department of Oral and Maxillofacial Pathology, Kalinga Institute of Dental Sciences, Campus 5, KIIT Deemed to be University, Bhubaneswar-751024, Odisha, India.
  • Dr. Vidya K.C., Department of Oral & Maxillofacial Surgery, Kalinga Institute of Dental Sciences, Campus 5, KIIT Deemed to be University, Bhubaneswar-751024, Odisha, India.

Lab Members

Shweta Chaturvedi, Ph.D Candidate

Shweta comes from Ranchi, Jharkhand. She has earned her Master of Science in Plant Biotechnology from Ranchi University. Shweta is working on studying the role of peroxisomal polyamine oxidase in abiotic stress tolerance in rice. She is using bioinformatics, molecular biology and biochemical tools to decipher the signal transduction pathway for peroxisomal PAO in rice and Arabidopsis.
E mail : [email protected]

 

Berhanu Girma Shewakena, Ph.D. Candidate

Berhanu comes from Ethiopia. He earned his Master of Science in Forestry and comes with six years of teaching experience. He is pursuing his doctoral research on isolation and characterization of plant growth promoting rhizobacteria and their role in abiotic stress adaptation using biochemical, molecular and bioinformatics tools. He is using rice as model for investigation. He is co-supervised by Dr. Sasmita Mohanty.
E mail: [email protected]

 

Sabiha Tarafdar, Junior Research Fellow

Sabiha comes from Bhubaneswar, Odisha. She has earned her master in Applied Microbiology from KIIT-DTU. Currently, she is working as junior research fellow. Her research area is tomato peroxisome proteome. She is using bioinformatics and molecular tools to identify novel peroxisomal proteins in tomato.
E mail: [email protected]

 

Shrutakirti Saha, Junior Research Fellow

Shrutakirti comes from Kolkata, West Bengal.  She has earned her M. Tech in Biotechnology from, KIIT-DTU. Currently she is working as junior research fellow. Her research area is Vigna peroxisome proteome. She is using bioinformatics and molecular tools to identify novel peroxisomal proteins in Vigna.
E mail: [email protected]

 

 

Pamela Chanda Roy, Master’s trainee

Pamela comes from Siliguri, West Bengal.  She is currently pursuing her dissertation for successful completion of her Master of Technology in Biotechnology from KIIT-DTU.  She is working on a novel peroxisomal glutathione reductase and trying to find out its interacting partners in rice.

 

 

Monalisa Patra, Master’s trainee

Monalisa comes from Bhubaneswar, Odisha.  She is currently pursuing her dissertation for successful completion of her Master of Science in Biotechnology from Rama Devi Women’s University.

 

Research Publications:

  • Gopal Chowdhary, Amr R A Kataya, Thomas Lingner, Sigrun Reumann (2012) Non-Canonical Peroxisome Targeting Signals: Identification of Novel PTS1 Tripeptides and Characterization of Enhancer Elements by Computational Permutation Analysis; BMC Plant Biol., 11; 12(1):142.
  • Gopal Chowdhary and Chanakya. Nath. Kundu (2012) Induction of a C-Repeat Binding Factor Gene by Calcium to resist Low Temperature stress in rice: Int. J. Plant Physiol. Biochem., (6) 142-145.
  • Renu Deswal, Gopal K. Chowdhary and Sudhir K. Sopory (2004) Purification and characterization of a PMA stimulated kinase and identification of PMA induced phosphorylation of a polypeptide that is dephosphorylated by low temperature in Brassica juncea,. Biochem. Biophys. Res. Commun., 322: 420-427.
  • S. Reumann and G. Chowdhary (2018): Prediction of Peroxisomal Matrix Proteins in Plants. Subcell Biochem.;89:125-138.

Conference proceedings / presentations:

  • Exploring Abiotic Stress Tolerance in Rice: The Peroxisome Perspective: at PerFuMe kick-off conference, December 1 – 4 2013, at Groningen, The Netherlands.
  • An Unexpected Snapshot of Protein Evolution: Peroxisomal Matrix Proteins of Higher Plants Are Evolving Secondary Targeting Signals: at Norwegian Plant Biology Conference, November 7 – 8 2013, Tromsø, Norway.
  • Peroxisome mediated mechanisms of abiotic stress tolerance: at Scandinavian Plant Physiology Society Congress, August 21- 25 2011, Organized by Center for Organelle Research, University of Stavanger, Stavanger, Norway.
  • C-repeat binding factor and its role in cold acclimation: at Emerging Trends in Biotechnology, October 24 – 26 2009, Organized by KIIT, India
  • Role of teachers in professional education in the changing scenario: at National Seminar, October 19 2008, Organized by KIIT, India.
  • Cloning and characterization of a C-repeat binding factor gene from Brassica juncea; at National Symposium on plant Biotechnology; “In vitro Regeneration & Cloning Techniques”, October 12-14, 2006, Organized by Forest Research Institute Dehdradun, India.
  • Partial purification and characterization of cold inducible enzyme calcineurin from Brassica junaca: at BioTech 2004 “Challenges & Opportunities”, Organized by Institute of Genomics and Integrative Biology, Delhi, India, October 13 – 15th 2004