Dr. Subrat Kumar
Assistant Professor
Ph.D. Post Graduate Institute of Medical Education and Research, Chandigarh, 2006
M.Sc. Orissa University of Agriculture and Technology, Bhubaneswar, 1999


Email : [email protected]

Postdoctoral Experience

  • University of Maryland, USA, 2008-2010
  • High Security Animal Disease Lab, Bhopal, India, 2007-2008

Profile

  • July 2011 to Present: Assistant Professor, School of Biotechnology, Orissa, INDIA
  • May 2008 to Dec 2010: Post Doctoral Associate, University of Maryland, USA
  • March 2007 to April 2008: Research Associate, High Security Animal Disease Laboratory, Bhopal, INDIA.
  • Jan 2002 to Dec 2006: PhD, Post Graduate Institute of Medical Research and Education, Chandigarh, INDIA
  • 1998 to 1999: MSc, Microbiology, Orissa University of Agriculture and Technology, Bhubaneswar, INDIA.
  • 1995 to 1997: BSc, Botany (Hons), Orissa University of Agriculture and Technology, Bhubaneswar, INDIA.

Research Interest

  • Molecular Characterization of New and Emerging Human Metapneumovirus Strains
  • Reverse Genetics platform to produce viral vaccine candidates
  • Identification of molecular determinants for Dengue Pathogenesis

Awards/Honours

  • My research work on hepatitis E virus was recognised at The International Society of Infectious Disease (ISID) and was awarded the INTERNATIONAL, NOVARTIS VACCINE AWARD IN EPIDEMIOLOGY OF INFECTIOUS DISEASES in March 2008 at Malaysia.
  • Dr. V. N. Bachhil Young Scientist Award at the International Symposium and Fifth Annual Conference on “New strategies for prevention and control of zoonosis: An integrated veterinary and medical approach” by Indian Association of Veterinary Public Health Specialists held at Palampur during 12-14 October, 2006.
  • First Prize for Best Poster presentation at the National Symposium conducted by Indian Virology Society at New Delhi during November 2004.
  • Selected as Research Fellow of PGIMER for the year 2002 (2002-2006).

Selected Publications

Full List
  1. Kumar S. Extinction need not be forever. Nature. 6 Dec 2012; Vol-492 (7427): 9.
  2. Panda S. Mohakud NK, Pena L, Kumar S*. Human metapneumovirus: Review of an important respiratory pathogen. International Journal of Infectious Diseases. August 2014; 25:45-52. (*Corresponding author)
  3. Kumar S, Subhadra S, Singh B and Panda BK. Hepatitis E virus: The current scenario. International Journal of Infectious Diseases. April 2013; 17 (4): e228-e233
  4. Kumar S, Pujhari SK, Chawala Y, Chakraborti A, Ratho RK. Molecular detection and sequence analysis of Hepatitis E Virus in patients with viral hepatitis from North India. Diagnostic Microbiology and Infectious Disease. Oct 2011; 71 (2):110-7.
  5. Kumar S, Ratho RK, Chawla YK and Chakraborti A. The incidence of sporadic viral hepatitis in North India: A Preliminary study. Hepatobiliary and Pancreatic Disease International. 2007 Dec; 6(6): 596-9

RNA viruses are responsible for some of the deadliest diseases in humans (Ebola, SARS, Influenza etc). As these RNA viruses lack a proof reading mechanism and keeps on mutating, developing a vaccine against them becomes a challenge. In order to develop an effective anti-viral strategy, it becomes imperative to study the pathogenesis of the virus. Although, live attenuated virus is a good candidate for vaccine development, the traditional process of attenuating can be long, tedious and sometime ineffective. With the emergence of emergence of plasmid based reverse genetics (PBRG) systems, manipulation of RNA viruses in lab have become easier. With the current advancements in reveres genetic system, it is now possible to (i) Identify the attenuating mutations in the virus, (ii) To develop additional, new types of attenuating mutations, including deletion of some non-essential genes and (iii) To construct recombinant vector that will act as vaccine candidate containing desired combinations of mutations. With the help of PBRG, it is now possible to carry out site specific mutations in the virus genome to generate a mutant strain to elucidate its role in disease severity and to use it as a live vaccine strain. Currently my lab is involved in elucidating molecular determinants of disease severity in Human metapneumovirus, Dengue virus and Hepatitis E Virus using PBRG systems.

Figure: Eight-plasmid system for rapid generation of reassortant influenza A virus. To test the utility of the eight-plasmid system for generation of reassortant viruses, 293T–MDCK cells were co-transfected with six plasmids representing the A/PR/8/34 (H1N1) master strain and two plasmids representing the desired HA and/or NA subtypes. Influenza viruses were generated within 2–3 days after transfection. (Reference: E. Hoffmann et al.  Vaccine 20 (2002) 3165–3170)

 

Projects

Immunological study of neurocysticercosis, and estimation of its prevalence among patients with epilepsy by serological diagnosis
Funding agency: ICMR, Govt of India

Peptide-stabilized nanoparticles and their potential antimicrobial and antiviral activities
Funding agency: DST, Govt of India

Collaborators

  • Dr. Narahari Agasti, Dept. of Obstetrics and Gynaecology, KIMS, KIIT, Bhubaneswar
  • Dr. Nirmal Kumar Mohakud, Dept. of Paediatrics, KIMS, KIIT, Bhubaneswar
  • Dr. Subhranshu Patro, Dept. of Medicine, KIMS, KIIT, Bhubaneswar
  • Dr. B Mishra, Dept. of Microbiology, AIIMS, Bhubaneswar
  • Dr. Ranjan Nanda, Immunology Group, ICGEB, New Delhi
  • Dr. Lindomar Pena, Federal University of Paraiba, Paraiba, Brazil

Ms Swagatika Panda (DST Inspire Fellow)
Email: [email protected]  
Project: Identification of molecular determinants of pathogenesis and development of a live attenuated vaccine candidate for Human Metapneumovirus.
Publications:
Panda S. Mohakud NK, Pena L, Kumar S. Human metapneumovirus: Review of an important respiratory pathogen. International Journal of Infectious Diseases. August 2014; 25C:45-52.
Hobbies: Reading, Listening to songs.

Publication

  1. Panda S. Mohakud NK, Suar M, Kumar S*. Etiology, seasonality and clinical characteristics of respiratory viruses in children with respiratory tract infections in Eastern India (Bhubaneswar, Odisha). Journal of Medical Virology. 10 Aug 2016 (Accepted). (*Corresponding author)
  2. Panda S. Mohakud NK, Pena L, Kumar S*. Human metapneumovirus: Review of an important respiratory pathogen. International Journal of Infectious Diseases. August 2014; 25C:45-52. (*Corresponding author)
  3. Subhadra S, Kumar S, Suryanarayana VVS, and Sreenivasulu D. Comparison of bluetongue virus detection and quantitation methods in South India. Journal of Infection in Developing Countries. Oct 2014; 15 (8): 1307-12.
  4. Kumar S, Subhadra S, Singh B and Panda BK. Hepatitis E virus: The current scenario. International Journal of Infectious Diseases. April 2013; 17 (4): e228-e233
  5. Kumar S. Extinction need not be forever. Nature. 6 Dec 2012; Vol-492 (7427): 9.
  6. Kumar S, Pujhari SK, Chawala Y, Chakraborti A, Ratho RK. Molecular detection and sequence analysis of Hepatitis E Virus in patients with viral hepatitis from North India. Diagnostic Microbiology and Infectious Disease. Oct 2011; 71 (2):110-7.
  7. Pujhari SK, Kumar S, Ratho RK, Chawala Y, Chakraborti A. Phylogenetic analysis and sub-typing of Acute and fulminant strains of Hepatitis E Virus Isolates of North India with reference to disease severity. Archives of Virology. Sep 2010; 155 (9): 1483-6.
  8. Bali S, Kar SS, Kumar S, Ratho RK, Dhiman RK and Kumar R. Hepatitis E Epidemic with Bimodal Peak in a Town of North India. Ind J Public Health. 2008 Oct-Dec; 52 (4):189-199.
  9. Murugkar HV, Nagarajan S, Tosh C, Bhatia S, Venkatesh G, Jain R, Kumar S, Khandia R, Pandey M, Behera P, Tripathy S, Kulkarni DD and Dubey SC. H5N1 Outbreaks in Poultry in India. Veterinary Record. February 23, 2008; Vol.162 (8): pp-255.
  10. Prinja S, Kumar S, Reddy GMM, Ratho RK and Kumar R. Investigation of viral hepatitis E outbreak in a town in Haryana. J Commun Dis. 2008; 40 (4): 249-254.
  11. Tosh C, Murugkar HV, Nagarajan S, Bhatia S, Pateriya AK, Behera P, Jain R, Kumar S, Khandia R, Vanamaya PR, Dubey SC and Ahlawat SPS. Outbreak of avian influenza virus H5N1 in India. Veterinary Record. August 25, 2007; Vol.161 (8): pp-279.
  12. Kumar S, Ratho RK, Chawla YK and Chakraborti A. The incidence of sporadic viral hepatitis in North India: A Preliminary study. Hepatobiliary and Pancreatic Disease International. 2007 Dec; 6(6): 596-9.
  13. Ratho RK, Mishra B, Kumar S and Verma S. Dengue fever (DF)/ dengue hemorrhagic fever (DHF) in Chandigarh (North India). (Short note) WHO Dengue Bulletin. 2006; Vol-30: 278-280.
  14. Bali S, Ratho RK, Kumar S and Singh A. Homosexuality as the mode of transmission of hepatitis E virus: A study in a North Indian village. Internet J of Epidemiology. (2006). Vol. 43.
  15. Kumar S, Ratho RK, Chawla YK and Chakraborti A. Virological investigation of an epidemic of Hepatitis E virus in North India. Singapore Med J. 2006; 47: 631-633.
  16. Kumar S, Panda HK, Kar BC and Panda BK. Adaptation and physicochemical characterization of MB strain of infectious Bursal disease virus. Ind. Vet. J. 2005; 82: 923-925.
  17. Kumar S, Panda HK and Panda BK. Biological characterization of the MB strain of IBD virus in chick embryo fibroblast cell culture. Ind. J. Poultry Sci.  2005; 40: 211-212.

Paper/Abstract presented in conferences

  1. Shrivastava A, Mahapatra R, Subrat Kumar, Sahu PS. “In-Silico prediction of therapeutic candidates in Cryptosporidium hominis”. 83rd National conference on evolution: Molecules to Life organized by Society of Biological Chemists of India (SBCI) held at Bhubaneswar, India during Dec 17- Dec 21 2014. (Poster presented by A. Shrivastava).
  2. Panda S, Mohakud NK, Subrat Kumar. “Epidemiological and clinical study on respiratory viruses found in children`s with respiratory tract infections in Eastern India”. 55th National conference on empowering mankind with Microbial Technologies organized by Association of Microbiologists of India (AMI) held at Coimbatore, India during Nov 12- Nov 14 2014. (Poster presented by S. Panda).
  3. Subrat Kumar. “Diagnostic assays in Virology”. Guest Lecture at State level Seminar on DNA sequencing, bioinformatics tools and microbial diagnostics organized by the plant pathology and microbiology division, RPRC, Bhubaneswar on 4th July 2013.
  4. Subrat Kumar. “Reverse Genetics approach in virology research”. Oral presentation in virology section at the 53rd International conference on Microbiology organized by Association of Microbiologists of India (AMI) held at Bhubaneswar during 22-25th November 2012.
  5. Lindomar Pena, Yonas Araya, Subrat Kumar, Matthew Angel, and Daniel R. Perez. “Surface glycoproteins modulate the lung pathology of triple reassortant H3N2 influenza viruses in swine”. Poster presented in microbiology and immunology section at the Bioscience Research and Technology Review Day, University of Maryland on November 9, 2010. (Poster presented by L. Pena).
  6. Pujhari SK, Ratho RK, Kumar S, Chawla YK and Chakraborti A. “Molecular epidemiology and sub-typing of hepatitis E virus (HEV) strains circulating in North India”. International Liver Congress, 2010, Vienna, Austria during 14-18 April 2010. (Poster presented by R.K. Ratho).
  7. Ratho RK, Kumar S, Chawla YK and Chakraborti A. “Role of ORF 1 polymorphism, viral load and cytokine response in the pathogenesis of acute and fulminant hepatitis E virus infection”. 13th International Congress of Infectious Diseases, Kuala Lumpur, Malaysia during 19-22 June 2008. (Oral presentation by R.K. Ratho).
  8. Kumar S, Ratho RK, Chawla YK and Chakraborti A. “Cytokine response in acute and fulminant Hepatitis E virus infection”. Seventh Asia-Pacific Congress of Medical Virology (APCMV-2006) held at New Delhi during 13-15 November 2006. (Oral presentation by S. Kumar).
  9. Kumar S, Ratho RK, Chawla YK and Chakraborti A. “Correlation between viral load and cytokine levels in acute and fulminant hepatitis E virus infection”. International Symposium and Fifth Annual Conference of Indian Association of Veterinary Public Health Specialists (IAVPHS-2006) held at Palampur during 12-14 October 2006. (Oral presentation by S. Kumar).
  10. Kumar S, Ratho RK, Chawla YK and Chakraborti A. “Th1 and Th2 cytokine response in HEV induced acute viral hepatitis patients”. XXXII Annual Conference of Indian Immunology Society IMMCON-2005 held at PGIMER Chandigarh during 24-27 November 2005. (Poster presented by S. Kumar).
  11. Kumar S, Ratho RK, Chawla YK and Chakraborti A. “Production of TNFα and IFNγ in acute and fulminant hepatitis E virus (HEV) patients”. National Symposium on Molecular Diagnostics for the Management of Viral Diseases held at Indian Agricultural Research Institute, New Delhi during 14-16 November 2004. (Poster presented by S. Kumar).
  12. Kumar S, Ratho RK, Chawla YK and Chakraborti A. “Epidemic of Hepatitis E in North India”. XXVII National Conference of Indian Association of Medical Microbiologists (IAMM) held at Mumbai during 5-9 November 2003. (Oral presentation by S. Kumar).